RESUMO
A polysaccharide (LVP) was purified from fruiting body of Lentinus velutinus by ethanol precipitationfractionation and DEAE and Sephadex G-100 column chromatography. The yieldof purified polysaccharide was 0.025%. Molecular characteristics of LVP were determinedby gel permeation chromatography, FT-IR spectroscopy, and thin-layer chromatography.Our results revealed that LVP is a polysaccharide composed of only glucose units, and hasa molecular weight of 336 kDa. Biological activity assays indicated that LVP exhibits bothcytotoxic and antioxidant activity. LVP showed specific cytotoxicity against cancer cells(HeLa and HepG2 cells), and alterations in cancer cell morphology were found afterLVP treatment.
RESUMO
A polysaccharide (LVP) was purified from fruiting body of Lentinus velutinus by ethanol precipitationfractionation and DEAE and Sephadex G-100 column chromatography. The yieldof purified polysaccharide was 0.025%. Molecular characteristics of LVP were determinedby gel permeation chromatography, FT-IR spectroscopy, and thin-layer chromatography.Our results revealed that LVP is a polysaccharide composed of only glucose units, and hasa molecular weight of 336 kDa. Biological activity assays indicated that LVP exhibits bothcytotoxic and antioxidant activity. LVP showed specific cytotoxicity against cancer cells(HeLa and HepG2 cells), and alterations in cancer cell morphology were found afterLVP treatment.
RESUMO
Aims@#The aim of this study was to evaluate some chemical properties and the cytotoxic effect of aqueous and ethanolic crude polysaccharides extracted from five Lentinus sp. on human cancer cell lines. It was hypothesized that other species of the genus Lentinus could show the pharmacological actions as presence in Lentinus edodes, especially anticancer properties. @*Methodology and results@#Crude extracts of dried fruit bodies and mycelia from L. edodes, Lentinus sajor-caju, Lentinus swartzii, Lentinus squarrosulus and Lentinus velutinus were extracted using two solvents, hot water and 95% ethanol, and evaluated for their total carbohydrates, proteins, reducing sugar, phenol contents, and cytotoxicity. The yield of crude extracts was 33.6-205.3 mg/g dry weight of a sample. Cytotoxicity was determined with 10 mg/mL of crude aqueous and 1 mg/mL of crude ethanolic extracts by using the [3-(4,5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide] (MTT) method. All extracts showed non-cytotoxicity against the normal cell lines, LLC-MK2 and L929 cells. While, the extracts of L. edodes, L. sajor-caju, L. squarrosulus and L. velutinus displayed the cytotoxicity against the human cancer cell lines. @*Conclusion, significance and impact of study@#The crude aqueous and ethanolic extract from fruit bodies of L. velutinus and the ethanolic extract from fruit bodies of L. sajor-caju and L. squarrosulus displayed the adverse effect against the human cancer cell lines. Hence, these extracts are a potential source of bioactive compounds for cancer treatment.